Glia cell stimulating factor (GSF): A new lymphokine
Identifieur interne : 000B64 ( Main/Exploration ); précédent : 000B63; suivant : 000B65Glia cell stimulating factor (GSF): A new lymphokine
Auteurs : A. Fontana [Suisse] ; R. Dubs [Suisse] ; R. Merchant [Suisse] ; S. Balsiger [Suisse] ; P. J. Grob [Suisse]Source :
- Journal of Neuroimmunology [ 0165-5728 ] ; 1981.
Abstract
The effect of activated-lymphocyte supernatant on glia cells was investigated. When treated in vitro with Concanavalin A (ConA), murine spleen cells released a soluble product, termed glia cell stimulating factor (GSF), which stimulated RNA and DNA synthesis in cultured murine glia cells. Furthermore, GSF appeared to promote the maturation of undifferentiated glia cells to astrocytes having a high content of glial fibrillary acidic protein. GSF secretion occurred after a lag period of 16 hours and proceeded at a constant rate for more than 48 hours. This GSF produced by ConA-stimulated murine lymphocytes has an apparent molecular weight between 60,000 and 80,000. Antigenic stimulation of primed lymph node cells with BGG resulted in a similar GSF production. Cellular sources of mitogen-induced GSF were investigated by using isolated lymphoid populations. GSF release by ConA-activated pure T-lymphocytes reconstituted with peritoneal macrophages was equivalent to that of unseparated spleen cells, whereas GSF production by T-lymphocytes alone was low. Macrophages alone did not elaborate detectable levels of GSF. GSF was also secreted by enriched B-lymphocytes populations stimulated by Protein A. Formation of GSF was suppressed when cytochalasin B or cyclo
Url:
DOI: 10.1016/0165-5728(82)90075-3
Affiliations:
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Fontana</name><affiliation wicri:level="1"><country xml:lang="fr">Suisse</country><wicri:regionArea>Department of Internal Medicine, Section of Clinical Immunology</wicri:regionArea><wicri:noRegion>Section of Clinical Immunology</wicri:noRegion></affiliation></author><author><name sortKey="Dubs, R" sort="Dubs, R" uniqKey="Dubs R" first="R." last="Dubs">R. Dubs</name><affiliation wicri:level="1"><country xml:lang="fr">Suisse</country><wicri:regionArea>Department of Internal Medicine, Section of Clinical Immunology</wicri:regionArea><wicri:noRegion>Section of Clinical Immunology</wicri:noRegion></affiliation></author><author><name sortKey="Merchant, R" sort="Merchant, R" uniqKey="Merchant R" first="R." last="Merchant">R. 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Grob</name><affiliation wicri:level="1"><country xml:lang="fr">Suisse</country><wicri:regionArea>Department of Internal Medicine, Section of Clinical Immunology</wicri:regionArea><wicri:noRegion>Section of Clinical Immunology</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Neuroimmunology</title><title level="j" type="abbrev">JNI</title><idno type="ISSN">0165-5728</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1981">1981</date><biblScope unit="volume">2</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="55">55</biblScope><biblScope unit="page" to="71">71</biblScope></imprint><idno type="ISSN">0165-5728</idno></series><idno type="istex">C7079CBF195318007D30B01A3BCF325570C21E5A</idno><idno type="DOI">10.1016/0165-5728(82)90075-3</idno><idno type="PII">0165-5728(82)90075-3</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0165-5728</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The effect of activated-lymphocyte supernatant on glia cells was investigated. When treated in vitro with Concanavalin A (ConA), murine spleen cells released a soluble product, termed glia cell stimulating factor (GSF), which stimulated RNA and DNA synthesis in cultured murine glia cells. Furthermore, GSF appeared to promote the maturation of undifferentiated glia cells to astrocytes having a high content of glial fibrillary acidic protein. GSF secretion occurred after a lag period of 16 hours and proceeded at a constant rate for more than 48 hours. This GSF produced by ConA-stimulated murine lymphocytes has an apparent molecular weight between 60,000 and 80,000. Antigenic stimulation of primed lymph node cells with BGG resulted in a similar GSF production. Cellular sources of mitogen-induced GSF were investigated by using isolated lymphoid populations. GSF release by ConA-activated pure T-lymphocytes reconstituted with peritoneal macrophages was equivalent to that of unseparated spleen cells, whereas GSF production by T-lymphocytes alone was low. Macrophages alone did not elaborate detectable levels of GSF. GSF was also secreted by enriched B-lymphocytes populations stimulated by Protein A. Formation of GSF was suppressed when cytochalasin B or cyclo</div></front></TEI><affiliations><list><country><li>Suisse</li></country><region><li>Canton de Zurich</li></region><settlement><li>Zurich</li></settlement></list><tree><country name="Suisse"><noRegion><name sortKey="Fontana, A" sort="Fontana, A" uniqKey="Fontana A" first="A." last="Fontana">A. Fontana</name></noRegion><name sortKey="Balsiger, S" sort="Balsiger, S" uniqKey="Balsiger S" first="S." last="Balsiger">S. Balsiger</name><name sortKey="Dubs, R" sort="Dubs, R" uniqKey="Dubs R" first="R." last="Dubs">R. Dubs</name><name sortKey="Grob, P J" sort="Grob, P J" uniqKey="Grob P" first="P. J." last="Grob">P. J. Grob</name><name sortKey="Merchant, R" sort="Merchant, R" uniqKey="Merchant R" first="R." last="Merchant">R. Merchant</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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